Clear space around the cooling vents. Clean dust off the condenser coils. Internal electronic or speed sensor fault.
Immediately press Stop . Check tube balancing using a digital laboratory balance. Ensure the central rotor nut is tightened properly using the original Eppendorf rotor wrench. Error Messages (Flashing Lights / Indicators)
Maintain a clearance safety zone of at least 30 cm (12 inches) around the entire unit.
Always use an aerosol-tight rotor when dealing with infectious or hazardous materials. eppendorf 5402 manual updated
Once the timer hits zero, the rotor brakes to a stop. Wait for the complete stop signal, press Open , unscrew the rotor lid, and retrieve your samples. 4. Routine Maintenance and Care
: The 5402 features adjustable speeds from 1,000 to 14,000 RPM (approx. 16,000 x g).
If you are using the original 1990s or early 2000s instruction booklet, you are likely missing critical updates. Since the centrifuge’s production ceased, Eppendorf has released several (TI sheets) and safety updates that supersede the original manual. Clear space around the cooling vents
The 5402 is designed for high-speed separation of small sample volumes, typically utilizing 1.5 mL and 2.0 mL Eppendorf tubes . Key technical specifications include: Speed Range: Variable speeds up to 14,000 RPM. Temperature Control:
Power failure or a jammed mechanical emergency release.
If a tube breaks, immediately power off the unit. Clean the rotor and chamber using a mild, neutral detergent. If hazardous bio-agents or radioactive materials are involved, use an approved laboratory decontaminant that does not corrode aluminum. Immediately press Stop
Toggle the main power switch located on the side or rear panel of the unit.
The 5402 was designed for high-speed separation of temperature-sensitive samples. 14,000 RPM (approx. 16,000 x g). Speed Increments: Adjustable in 100 RPM steps. Temperature Range: -9°C to +40°C.
The original manual required complex menu diving. The updated quick-reference suggests:
Opposing tubes must be of equal weight, volume, and density.